Through coordinating organic ligands, metal particles, template, and controlling reaction conditions, organic skeleton materials of porous metal will be likely produced. 通过调节有机配体、金属离子、模板剂和控制反应条件可能定向组装出预期的多孔金属有机骨架材料。
Preparation of Single-Stranded DNA Template for Pyrosequencing by Linear-after-the-Exponential-Polymerase Chain Reaction 指数线性聚合酶链式反应法制备焦磷酸测序反应的单链模板
The anomalous typing could result from a lot of causes, including DNA genetic variation, poor quality or quantity of DNA template, different typing system or method, nonspecific reaction in PCR or anomalous electrophoresis migration. 引起这些异常现象原因很多,有DNA的遗传性变异、DNA模板质量不好、分型试剂或方法的不同、PCR反应中非特异性反应以及异常的电泳行为等。
Results For LCN template DNA, if only increased the amount of Taq enzyme or the reaction volume, the results of STR amplification were scarcely improved, but if increased PCR cycles, the detection sensitivity were significantly improved. 结果对低拷贝模板DNA,单纯增加Taq酶量或反应体系,扩增效率改善不明显;增加循环数,显著提高检验灵敏度;
This model describes the function relation between product quantity or fluorescence intensity and initial template number and other reaction conditions, and can reflect the accumulating rule of PCR product molecule accurately. PCR动力学数学模型是根据PCR技术的原理提出的,能够准确描述PCR反应产物分子数量积累规律的动力学方程,给出了PCR产物数量或者荧光强度与初始模板数量及其他反应条件间的函数关系。
Applied FeSO_4 · 7H_2O and ( NH_4)_3PO_4 · 3H_2O as materials, PEG-400 as template, multi-micronutrient fertilizer ferrous ammonium phosphate was synthesized by low heat solid state reaction. 以FeSO4.7H2O和(NH4)3PO4.3H2O为原料,用聚乙二醇-400作模板剂,经低热固相反应合成了磷酸亚铁铵多元微肥。
A novel method for synthesis of sodium aluminium carbonate hydroxide was studied, and the target product was obtained with aluminium sulfate as aluminium source and PEG-400 as template via the one step low heat solid state reaction. 对合成碱式碳酸钠铝的新方法进行了研究,以聚乙二醇400(PEG400)为模板,硫酸铝为铝源,用低热固相反应一步法成功合成得到碱式碳酸钠铝。
Using the DNA extracted from bone marrow smears which have been stored routinely as a template, polymerase chain reaction ( PCR) was utilized to detect clonal immunoglobulin heavy chain ( IgH) gene rearrangements in multiple myeloma ( MM) and reactive plasmacytosis ( RP). 从常规保存的骨髓涂片抽提DNA为模板,应用聚合酶链反应(PCR)技术检测多发性骨髓瘤(MM)与反应性浆细胞增多症(RP)IgH基因重排方式。
The effect of different concentrations of template DNA, primer, dNTPs and Mg2+ on the PCR reaction was analyzed and the optimized RAPD and ISSR-PCR systems in radish were established. 对影响PCR反应的主要因子&模板DNA、引物、dNTPs和Mg2+浓度进行优化,建立起适合于萝卜基因组DNA的RAPD和ISSR反应体系。
A simple and rapid method was used to prepare template DNA for polymerase chain reaction, Cells and minced tissues were digested with proteinase K. 在多聚酶链反应时采用一种快速简便的模板DNA制备方法。组织块在冰浴中剪碎和匀浆,再用蛋白酶K降解;
S A method of rapid detecting Leptospira DNA was constructed after the template DNA from 4 serotypes of Leptospira interrogans were successfully amplified by polymerase chain reaction ( PCR). 以四株不同型别的致病性钩端螺旋体DNA为模板进行聚合酶链反应(PCR),建立了钩体快速检测方法。
Recombinant PUC inserted with full length nucleic acid sequence of chrysanthemum stunt viroid was used as template DNA to prepare digoxigenin-labelled and biotin-labelled probes with two universal primers by polymerase chain reaction. 以含菊花矮化类病毒(Chrysanthemumstuntviroid.CSV)全长序列的重组质粒PUC为模板DNA,加入通用引物,在聚合酶链式反应系统中制备***标记的CSVcDNA探针和生物素标记的CSVcDNA探针。
The results showed that the insert fragment length of target clones as λ phage DNA template directly sequencing was longer than that of PCR products after recycling, but a lower succeeding ratio for sequencing reaction. 结果表明,以λ噬菌体DNA为模板直接测序比PCR产物经回收后为模板进行测序,其目标克隆的平均插入片段长度要长,但反应成功率低。
A rapid, simple and reliable method to prepare dsRNA template for reverse transcription polymerase chain reaction is established. 应用RT-PCR技术检测草鱼出血病病毒。建立了一种快速、简易而可靠的dsRNA模板的制备方法。
Methanol method for the preparation of template DNA from blood for polymerase chain reaction 甲醇法制备的血模板DNA用于聚合酶链反应
Then the positive TTV DNA was used as a template to optimize nested PCR reaction conditions. The methods were verified through repetition, sensitivity and specificity of tests. 再用阳性TTVDNA为模板,对巢式PCR反应条件进行优化,通过重复性、敏感性、特异性试验来验证该方法的准确性。
Used this as a template, the endoglucanase cellulose was cloned through PCR reaction. 2. 以此为模板,通过克隆测序得到纤维素葡聚糖内切酶。
Acetonitrile as solvent, when the molar ratio of template, functional monomer and cross linker is1:8:8and the reaction temperature is60 ℃, the imprinted polymers possess optimal selectivity and adsorbability. 以乙腈为溶剂,模板、功能单体和交联剂的物质的量比为1:8:8时,反应温度为60℃时制备的印迹聚合物具有最佳的选择性、吸附性。
Also attended with a hard template to the reaction mixture, the hierarchical materials containing micro-, meso-and macropores were obtained after completing the reaction and removing the hard template. 向反应中加入硬模板,反应完成后洗脱模板,得到了同时含有微孔、介孔和大孔的多级孔材料。
The major results are as follows: 1. Compare the different effects of the dosage of template DNA, Mg2+, dNTPs and Taq enzyme in PCR amplification, then establish an ideal PCR reaction system to test cucumber hybrids purity. 2. 主要研究结果如下:1.比较模板DNA、Mg2+、dNTPs和Taq酶的不同用量对PCR扩增的影响,建立了适用于黄瓜杂种纯度检测的理想PCR反应体系。
In this part, a novel fluorescent method of protein detection has was developed using hairpin aptamer based on polymerase reaction. The hairpin aptamer was designed to be employed as the protein ligand and template of the polymerase reaction. 本部分设计了一种新型的发夹型核酸适体探针,利用其与靶蛋白反应后构象发生变化的特点,建立了一种基于聚合酶反应的荧光信号放大的蛋白质检测新方法。
CD was cultured successfully, and the genome was extracted as template of PCR reaction. 2. 获得如下结果;1.成功的培养了艰难梭菌,并提取了其基因组作为PCR反应模板。
And the catalysts for the reaction were optimized through comparison of experimental template reaction. 并通过对模板反应的对比实验,对反应中加入的催化剂进行了优化。
The results showed that: the template method combined with ultrasonic in two steps. The reaction was at 60 ℃ under ultrasonic for 3h, and molar ratio of catechol to 1, 3-dibromo propane was 1:1. 结果表明:用超声波法与模板法结合分两步加料,在60℃的反应条件下超声波反应3h,采用邻苯二酚同1,3-二溴丙烷总的摩尔比为1:1分两步加料。
Then the template substrate reaction with different boric acid, boric acid and ester reaction of pyridine, the corresponding yield obtained to study the law. 以此优化的条件为前提,再将该模板反应的底物与不同的硼酸,吡啶硼酸和酯反应,得出相应的产率,研究其规律。
Using potassium titanium oxalate as raw material, ring-like TiO2-aggregates film deposited on the glass substrate was successfully synthesized by hydrothermal method though a bubble template strategy and ligand exchanging reaction. 采用水热法,以草酸钛钾为反应原料,借助气泡模板和配体交换反应,在玻璃基底上成功沉积了环状的Ti02聚集体薄膜。